LOCUS       Exported               10050 bp DNA     circular SYN 21-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   John Yong
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3185..5149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    569..921
                     /label=Gag
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1311..1805
                     /label=env
     misc_feature    1806..1922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1988..2657
                     /label=UCOE
     primer_bind     2620..2637
                     /label=4-64_UCOE-midseq-F
     primer_bind     2707..2756
                     /label=4-52_NheI-EF1a-GF
     promoter        2756..3163
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     3088..3111
                     /label=3-72_SFFV-seq-F
     primer_bind     3132..3188
                     /label=8-28_SFFV-TagBFP-GF
     regulatory      3179..3188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3185..3274
                     /codon_start=1
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     primer_bind     complement(3255..3297)
                     /label=004_backbone_R
     primer_bind     3255..3296
                     /label=001_scarlet_F
     CDS             3275..3970
                     /codon_start=1
                     /label=IDTyomScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     misc_feature    3704..3718
                     /label=strand2
     CDS             3758..3766
                     /label=strand1
     primer_bind     3836..3856
                     /label=007_Scarlet_C_F
                     /note="sequencing primer to read forward from near the C 
                     terminus of Scarlet"
     misc_feature    3863..3875
                     /label=strand3
     CDS             3920..3922
                     /label=strand4
     primer_bind     3945..3987
                     /label=005_LAMP1_F
                     /note="for generating pTwist-Scarlet-LAMP1"
     primer_bind     complement(3945..3987)
                     /label=002_scarlet_R
                     /note="For generating pTwist_Scarlet_LAMP1"
     misc_feature    3971..3988
                     /label=phlare_linker
     CDS             3989..5146
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=rat lamp1
                     /translation="APALFEVKDNNGTACIMASFSASFLTTYEAGHVSKVSNMTLPASA
                     EVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTTRYSVQHMYFTYNLSDTQFFPN
                     ASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTIVLWDATIQAYLPSSNFSKEET
                     RCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGTCLLASMALQLNITYMKKDNTT
                     VTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFGMNATSSLFFLQGVQLNMTLPD
                     AIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKALALNVFSVQVQAFRVESDRFGS
                     VEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKRSHAGYQTI"
     misc_feature    5104
                     /label=C->T silent
     primer_bind     5127..5149
                     /label=003_backbone_F
                     /note="for generating pTwist_Scarlet_LAMP1"
     primer_bind     complement(5127..5149)
                     /label=006_LAMP1_R
                     /note="For generating pTwist_Scarlet_LAMP1"
     primer_bind     5157..5169
                     /label=4-52_NheI-EF1a-GF
     promoter        5175..5667
                     /label=EF-1-alpha promoter
     promoter        5175..5386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(5237..5256)
                     /label=1-41_EF1a-5'-seq-R
     primer_bind     complement(5368..5386)
                     /label=4-54_EFS-AbsI-loxP-GR
     LTR             5399..5667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     5579..5596
                     /label=1-31_EF1a-seq-F
     primer_bind     5655..5740
                     /label=1-1_AvrII-Blast-5'-GF
     misc_feature    5695..5700
                     /label=Inserted AvrII
     misc_feature    5701..5710
                     /label=Kozak sequence
     CDS             5713..6132
                     /codon_start=1
                     /label=Blasticidin resistance
                     /translation="KTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIISA
                     VHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCRE
                     LISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     primer_bind     5713..5740
                     /label=Blast-5'-primerbind
     primer_bind     complement(6108..6178)
                     /label=1-2_Blast-BsiWI-3'-GR
     primer_bind     complement(6108..6132)
                     /label=Blast-3'-primerbind
     misc_feature    6133..6138
                     /label=Inserted BsiWI
     misc_feature    6145..6733
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6220..6244)
                     /label=3-32_WPRE-seq-R
     CDS             complement(6616..6627)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6807..7040
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7112..7233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7149..7168)
                     /label=SV40pA-R
     rep_origin      7252..7387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7425..7441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7449..7465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7473..7503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7518..7539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7827..8415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8586..9446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9447..9551)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10025..10041
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     1921 ttatcgatac tagtattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1981 catctacggg aggtggtccc tgcagttacg ccaatgataa cccccgccag aaaaatctta
     2041 gtagccttcc ctttttgttt tccgtgcccc aactcggcgg attgactcgg ccccttccgg
     2101 aaacacccga atcaacttct agtcaaatta ttgttcacgc cgcaatgacc cacccctggc
     2161 ccgcgtctgt ggaactgacc cctggtgtac aggagagttc gctgctgaaa gtggtcccaa
     2221 aggggtacta gtttttaagc tcccaactcc ccctccccca gcgtctggag gattccacac
     2281 cctcgcaccg caggggcgag gaagtgggcg gagtccggtt ttggcgccag ccgctgaggc
     2341 tgccaagcag aaaagccacc gctgaggaga ctccggtcac tgtcctcgcc ccgcctcccc
     2401 cttccctccc cttggggacc accgggcgcc acgccgcgaa cggtaagtgc cgcggtcgtc
     2461 ggcgcctccg ccctccccct agggccccaa ttcccagcgg gcgcggcgcg cggcccctcc
     2521 ccccgccggg cgcgcgcccg ctgccccgcc cttcgtggcc gcccggcgtg ggcggtgcca
     2581 cccctccccc cggcggcccc gcgcgcagct cccggctccc tcccccttcg gatgtggctt
     2641 gagctgtagg cgcggagggc cgcctgcagc cccgataaaa taaaagattt tatttagtct
     2701 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag ctgcagtaac
     2761 gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc
     2821 gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc
     2881 cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga
     2941 acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt
     3001 ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct
     3061 gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc
     3121 ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggatctagc cctcgagggc
     3181 caccatggac agcaaaggtt cgtcgcagaa agggtcccgc ctgctcctgc tgctggtggt
     3241 gtcaaatcta ctcttgtgcc agggtgtggt ctccatggtc tctaagggtg aagccgtaat
     3301 taaggagttc atgagattca aagtccacat ggaaggttca atgaacggac atgagttcga
     3361 gatagaagga gagggggagg ggagacctta tgaggggacc cagacggcca agttgaaagt
     3421 gacgaaggga ggtccattgc ccttttcctg ggacatactg agtccccaat tcatgtatgg
     3481 aagccgtgcg tttacaaaac accccgctga tatccctgac tactataaac aatctttccc
     3541 ggaaggcttc aagtgggaaa gagtgatgaa tttcgaggac ggcggggcgg ttacggtgac
     3601 tcaggacact tcccttgaag acgggacctt aatttataaa gtaaaattga gggggacgaa
     3661 ctttcccccc gacggtccag taatgcaaaa aaaaacaatg ggctgggaag cctctactga
     3721 aaggttgtat cccgaagacg gcgtgttaaa gggagatatt aagatggctt taaggctaaa
     3781 ggacgggggg aggtatctgg cggattttaa aaccacatac aaggccaaga aaccggtcca
     3841 aatgccgggc gcttacaatg tcgacaggaa acttgatata acaagtcata atgaagacta
     3901 taccgttgtc gagcaatatg aaaggtcaga agggaggcac tctacagggg gaatggacga
     3961 gctatataag tccggatcga gcgccaccgc cccagcactg ttcgaggtga aagacaacaa
     4021 cggcacagcg tgtataatgg ccagcttctc tgcctccttt ctgaccacct atgaggctgg
     4081 acatgtttct aaggtctcga atatgaccct gccagcctct gcagaagtcc tgaagaatag
     4141 cagctcttgt ggtgaaaaga atgcttctga gcccaccctc gcaatcacct ttggagaagg
     4201 atatttactg aaactcacct tcacaaaaaa cacaacacgt tacagtgtcc agcacatgta
     4261 tttcacatat aacctgtcag acacacaatt ctttcccaat gccagctcca aagggcccga
     4321 cactgtggat tccacaactg acatcaaggc agacatcaac aaaacatacc gatgtgtcag
     4381 cgacatcagg gtctacatga agaatgtgac cattgtgctc tgggacgcta ctatccaggc
     4441 ctacctgccg agtagcaact tcagcaagga agagacacgc tgcccacagg atcaaccttc
     4501 cccaactact gggccaccca gcccctcacc accacttgtg cccacaaacc ccagtgtgtc
     4561 caagtacaat gtgactggtg acaatggaac ctgcctgctg gcctctatgg cactgcaact
     4621 caacatcacc tacatgaaga aggacaacac gactgtgacc agagcattca acatcaaccc
     4681 aagtgacaaa tatagtggga cttgcggtgc ccagttggtg accctgaagg tggggaacaa
     4741 gagcagagtc ctggagctgc agtttgggat gaatgccact tctagcctgt ttttcctgca
     4801 aggagttcag ttgaacatga ctcttcctga tgccatagag cccacgttca gcacctccaa
     4861 ctattccctg aaagctcttc aggccagtgt cggcaactca tacaagtgca acagtgagga
     4921 gcacatcttt gtcagcaagg cgctcgccct caatgtcttc agcgtgcaag tccaggcttt
     4981 cagggtagaa agtgacaggt ttgggtctgt ggaagagtgt gtacaggacg gtaacaacat
     5041 gctgatcccc attgctgtgg gcggggccct ggcagggctg gtcctcatcg tcctcatcgc
     5101 ctatctcatc ggcaggaaga ggagtcacgc gggctatcag accatctaat gcgatcgctc
     5161 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5221 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5281 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5341 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     5401 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     5461 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     5521 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     5581 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     5641 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgt cgaccctagg
     5701 gccaccatgg gaaaaacctt caacatctct cagcaggatc tggagctggt ggaggtcgcc
     5761 actgagaaga tcaccatgct ctatgaggac aacaagcacc atgtcggggc ggccatcagg
     5821 accaagactg gggagatcat ctctgctgtc cacattgaag cctacattgg cagggtcact
     5881 gtctgtgctg aagccattgc cattgggtct gctgtgagca acgggcagaa ggactttgac
     5941 accattgtgg ctgtcaggca cccctactct gatgaggtgg acagatccat cagggtggtc
     6001 agcccctgtg gcatgtgtag agagctgatc tctgactatg ctcctgactg ctttgtgctc
     6061 attgagatga atggcaagct ggtcaaaacc accattgagg aactcatccc cctcaagtac
     6121 accaggaact aacgtacggt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6181 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6241 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6301 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6361 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6421 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6481 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6541 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6601 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6661 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6721 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6781 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     6841 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6901 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6961 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7021 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7081 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7141 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7201 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7261 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7321 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7381 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     7441 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7501 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7561 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7621 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7681 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8521 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8581 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8641 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8701 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8761 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8821 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8881 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8941 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9001 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9061 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9121 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9181 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9241 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9301 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9361 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9421 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9481 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9541 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9601 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9661 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9721 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9781 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9841 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     9901 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9961 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10021 cgttgtaaaa cgacggccag tgccaagctg
//
